A little something about SDS-PAGE. I’ve pulled out some protocol details and left the ideas. Hope everything’s clear.
Working on the western blot bit, it’s taking longer to draw than I thought!
I have not been around this whatnot in about a year exactly, ha. Gosh, I can’t wait to get back into a lab.
Bypass Commands From the Brain to Legs Through a Computer
Read the full article Bypass Commands From the Brain to Legs Through a Computer at NeuroscienceNews.com.
Gait disturbance in individuals with spinal cord injury is attributed to the interruption of neural pathways from brain to the spinal locomotor center, whereas neural circuits locate below and above the lesion maintain most of their functions. An artificial connection that bridges the lost pathway and connects brain to spinal circuits has potential to ameliorate the functional loss. A Japanese research group, led by Shusaku Sasada and Yukio Nishimura, has successfully made an artificial connection from the brain to the locomotion center in the spinal cord by bypassing with a computer interface. This allowed subjects to stimulate the spinal locomotion center using volitionally-controlled muscle activity and to control walking in legs.
The research is in Journal of Neuroscience. (full access paywall)
Research: “Volitional Walking via Upper Limb Muscle-Controlled Stimulation of the Lumbar Locomotor Center in Man” by Syusaku Sasada, Kenji Kato, Suguru Kadowaki, Stefan J. Groiss, Yoshikazu Ugawa, Tomoyoshi Komiyama, and Yukio Nishimura in Journal of Neuroscience. doi:10.1523/JNEUROSCI.4674-13.2014
Image: When turning off the computer-aided spinal cord bypass, the lower extremities which were in a relaxed state did not move even if the subject was swinging his/her arms. With the bypass turned on, when the subject swung his/her arms by his/her own will and a walking motion of the lower extremities began in rhythm to the motion of the arms. Credit Yukio Nishimura.
This image shows an artificial connection that connects brain to spinal circuits. Credit Yukio Nishimura.
set of nostalgia drawings by gabriel picolo. i don’t think i have enough space on my tumblr for all his works that i’d like to post.
these are incredible
i feel like we’re advancing too fast as a species
I lost my shit for like an hour
I got a migraine and threw up because of this
i cant sleep cause of this now
i feel…i dont feel real anymore
i usually use the spread brush for lines and then i mess around with the blending modes, using either brush or marker, more often the brush :B
i use low blending to blend the color in quicker with fewer strokes sometimes or when it’s in tiny spots
and i sometimes use the flat brushes that make it a bit more blocky
then it’s a bit of messing with the settings to find whats a good feel for ur brushes and whatnot
im not that good with explanations but i hope it helped somehow?? :’0